Postabsorption neutralization of poliovirus
نویسندگان
چکیده
منابع مشابه
Poliovirus neutralization test with poliovirus pseudovirus to measure neutralizing antibody in humans.
We complement Arita et al. (1) for their development of a safe, simple, and rapid test, employing poliovirus pseudovirus (generating luciferase signals) instead of the wild poliovirus, to measure the poliovirus neutralizing antibody titer in human serum. Apart from the end game of the global poliomyelitis eradication program, poliovirus pseudovirus technology (1) would be advantageous in the su...
متن کاملAntigenic variation and resistance to neutralization in poliovirus type 1.
Mutations have been identified in variants of poliovirus, type 1 (Mahoney) on the basis of their resistance to neutralization by individual monoclonal antibodies. The phenotypes of these variants were defined in terms of antibody binding; the pattern of epitopes expressed or able to be exploited for neutralization were complex. Single amino acid changes can have distant (in terms of linear sequ...
متن کاملDevelopment of a poliovirus neutralization test with poliovirus pseudovirus for measurement of neutralizing antibody titer in human serum.
In the Global Polio Eradication Initiative, laboratory diagnosis plays a critical role by isolating and identifying poliovirus (PV) from the stool samples from acute flaccid paralysis (AFP) cases. In recent years, reestablishment of PV circulation in countries where PV was previously eliminated has occurred because of decreased herd immunity, possibly due to poor vaccination coverage. To monito...
متن کاملEvidence for a complex structure of neutralization antigenic site I of poliovirus type 1 Mahoney.
We have selected neutralization escape mutants by using a monoclonal antibody (nt-MAb) against a sequential epitope between amino acids 93 through 104 (neutralization antigenic site I) of poliovirus type 1 Mahoney. The majority of mutants were also resistant against five strain-specific nt-MAbs which recognized conformation-dependent epitopes, suggesting that the neutralization antigenic site I...
متن کاملCharacterization of Poliovirus Neutralization Escape Mutants of Single-Domain Antibody Fragments (VHHs).
To complete the eradication of poliovirus and to protect unvaccinated people subsequently, the development of one or more antiviral drugs will be necessary. A set of five single-domain antibody fragments (variable parts of the heavy chain of a heavy-chain antibody [VHHs]) with an in vitro neutralizing activity against poliovirus type 1 was developed previously (B. Thys, L. Schotte, S. Muylderma...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1993
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.67.6.3126-3133.1993